UniProt Accession Number of Target Protein: Q8J0P4

Alternative Name(s) of Target: Asp f 9; Aspf9; probable glycosidase crf1; Crh-like protein 1; glucanase; allergen: Asp f 9; allergen Asp f 9Immunogen: 5C6 antibody was raised using a novel technique: mononuclear cells were taken from the human subjects with allergic bronchopulmonary aspergillosis and then screened for IgE secretion. Subsequently, they were used to generate hybridomas of which some were secreting anti-Asp f 9 IgE.

Specificity: This antibody recognises Asp f 9.

Application Notes: A target of the 5C6 antibody (Asp f 9) was confirmed through IP and mass spectrometry (Wurth et al., 2018). ELISA: this antibody detected Asp f 9 in a commercial Aspergillus extract ALKB in ELISA (Wurth et al., 2018). WB: 5C6 antibody was successful in detecting Asp f 9 in western blot samples from Aspergillus extracts HS and AlgryL (Wurth et al., 2018).

Antibody first published in:Wurth et al.Human IgE mAbs define variability in commercial Aspergillus extract allergen compositionJCI Insight. 2018 Oct 18;3(20). pii: 123387. doi: 10.1172/jci.insight.123387.PMID:30333320Note on publication:The article describes a method of generating Aspergillus-reactive IgE–secreting human hybridomas (among them these secreting this antibody). Subsequently, the antibody is characterized and its specificity is determined. Finally, this antibody is used to determin a potential presence of its antigen in the commercialy-available Aspergillus samples used in diagnosis and treatment of Aspergillus-related allergies.