Immunogen: The VHH-7a was generated by immunizing a dromedary with poliovirus type I Sabin strain, preparing cDNA libraries from lymphocytes, and then selecting for tight poliovirus binders by phage display.
Specificity: VHH-7a binds to a site on the top surface of the expanded poliovirion capsid protein VP3. VHH-7a does not bind to the native, unexpanded virus.
Application Notes: ELISA was used to show the reactivity of VHH-7a for poliovirus (Thys et al, 2010). The interaction of VHH-7a with capsid protein VP3 has been captured using cryo electron microscopy (Strauss et al, 2017).
Antibody first published in:Thys et al, 2010.In vitro antiviral activity of single domain antibody fragments against poliovirusAntiviral Res. 2010 Aug;87(2):257-64PMID:20566349Note on publication:Describes the generation of VHH-7a from a phage display library and its characterisation using ELISA.