UniProt Accession Number of Target Protein: P51681

Alternative Name(s) of Target: C-C chemokine receptor type 5; C-C CKR-5; CC-CKR-5; CCR-5; CHEMR13; HIV-1 fusion coreceptor; CD195; B8LFP0Immunogen: This antibody was isolated from a phage displayed antibody fragment library. The library was generated from a New Zealand White rabbit immunised with purified CCR5-N-GST protein, and four rounds of panning were completed against immobilised CCR5-N-MBP antigen.

Specificity: This antibody is specific for the YTSE region of the N-terminal extracellular domain of human CCR5.It also binds to rhesus CCR5.

Application Notes: When expressed intracellularly, this antibody is able to inhibit the surface expression of human CCR5, conferring T-lymphocytes with immunity to CCR5-dependent HIV-1 infection (Steinberger et al, 2000). Humanised ST6 (ST6/34) also has the same depleting effect (Steinberger et al, 2000). The high affinity of this antibody for CCR5-N-MBP has been confirmed in surface plasmon resonance analysis (Steinberg et al, 2000). This antibody has been used to stain CCR5-expressing cells in flow cytometry, with no staining of control cells observed (Steinberger et al, 2000).

Antibody first published in:Steinberger et al.Generation and Characterization of a Recombinant Human CCR5-specific Antibody: A PHAGE DISPLAY APPROACH FOR RABBIT ANTIBODY HUMANIZATIONJ Biol Chem. 2000 Nov 17;275(46):36073-8.PMID:10969070Note on publication:Describes the generation of this antibody by phage display, and its use in FC and ELISA.