Alternative Name(s) of Target: double-stranded RNAImmunogen: The original J2 antibody was raised by immunising mice with L-dsRNA.
Specificity: This antibody binds to dsRNA > 40 bp in length.
Application Notes: This recombinant mouse monoclonal antibody, rJ2, is useful for detecting double stranded RNA of greater than 40 bp in length which is generated during the replication of positive sense genome viruses. It works with HCV, rhinovirus, dengue virus, and others.It does not work for dsRNA produced by influenza A virus and other negative sense genome viruses.
Antibody first published in:J Schönborn, J Oberstrass, E Breyel, J Tittgen, J Schumacher, and N LukacsMonoclonal antibodies to double-stranded RNA as probes of RNA structure in crude nucleic acid extracts.Nucleic Acids Res. 1991 Jun 11; 19(11): 2993–3000PMID:2057357Note on publication:Describes the original generation and characterisation of this antibody.