![absoluteantibody/Anti-ICOSL [HK5.3]/200 μg/Ab00882-2.3](https://www.ebiomall.cn/images/no_picture.gif)
UniProt Accession Number of Target Protein: Q9JHJ8
Alternative Name(s) of Target: B7RP-1; B7-related protein 1; ICOS ligand; CD275; LICOS; B7hImmunogen: This antibody was raised byimmunizing SD rat with mouse B7h-transfected L cells and fusing immune splenocytes with P3U1 myeloma cells and screened for binding to mouse B7h-transfected NRK cells.
Specificity: This antibody specifically binds to mouse ICOSL and competes with binding of ICOS.
Application Notes: This antibody has been used to detect murine ICOSL by flow cytometry. It is able to block ICOSL interacting with ICOSL and inhibit T-cell proliferation in a collagen type II (CII)-induced arthritis model.
Antibody first published in:Iwai et al.Amelioration of collagen-induced arthritis by blockade of inducible costimulator-B7 homologous protein costimulation.J Immunol. 2002 Oct 15;169(8):4332-9PMID:12370365Note on publication:Describes the generation and initial characterisation of this antibody.
Immunofluorescence staining of fixed mouse splenocytes with anti-ICOSL (inducible T-cell costimulator ligand) antibody HK5.3 (Ab00882) Immunofluorescence analysis of paraformaldehyde fixed mouse (Mus musculus) splenocytes on Shi-fix™ coverslips, permeabilized with 0.15% Triton stained with the chimeric rabbit version of HK5.3 (Ab00882-23.0) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (1 µg/ml), showing weak membrane staining in a subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom Ab00882-23.0, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody (Ab102-23.0) followed by Alexa Fluor® 488 secondary antibody.
Binding curve of anti-ICOSL antibody HK5.3 (Ab00882-23.0) to mouse ICOSL-Fc fusion protein ELISA Plate coated with ICOSL-Fc fusion protein (Pr00157-1.9) at a concentration of 5 µg/ml. A 3-fold serial dilution from 10,000 to 0.1 ng/ml was performed using Ab00882-23.0. For detection, a 1:4000 dilution of HRP-labelled goat anti-rabbit antibody (Bio-Rad) was used.
