![绝对抗体/抗PD-L2[TY25]/100μg/Ab00883-1.32](https://www.ebiomall.cn/images/no_picture.gif)
UniProt Accession Number of Target Protein: Q9WUL5
Alternative Name(s) of Target: B7-DC; PDL2; B7DC; CD273; programmed death ligand 2; CD273; Pdcd1lg2Immunogen: This antibody was raised by immunising SD rats with B7-DC-transfected RAW264.7 cells and selected on B7-DC-transfected NRK.
Specificity: This antibody binds to mouse PD-L2 and competes with PD-1 binding. It does not cross-react with PD-L1.
Application Notes: This antibody has been used to detect murine PD-L2by flow cytometry on stimulated dendritic cells as well as various mouse tumour cells. It also detects immunoprecipitates mouse PD-L2 and can be used to detect it by non-reducing Western Blot. TY25 inhibits PD-L2 binding to PD-1 transfected cells (Ansari et al. 2003, PMID: 12847137) and thereby blocks PD-L2 : PD-1 signalling.
Antibody first published in:Yamazaki et al.Expression of programmed death 1 ligands by murine T cells and APC.J Immunol. 2002 Nov 15;169(10):5538-45PMID:12421930Note on publication:Describes the generation and initial characterisation of this antibody.
Binding curve of anti-PDL2 antibody TY25 (Ab00883-23.0) to mouse PDL2-Fc fusion protein ELISA Plate coated with mouse PDL2-Fc fusion protein (Pr00156-1.9) at a concentration of 5 µg/ml. A 3-fold serial dilution from 10,000 to 0.1 ng/ml was performed using Ab00883-23.0. For detection, a 1:4000 dilution of HRP-labelled goat anti-rabbit antibody (Bio-Rad) was used.
