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absoluteantibody/Anti-CD3 epsilon [OKT-3 (muromonab)]/50 μg/Ab00122-21.0
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UniProt Accession Number of Target Protein: P07766
Alternative Name(s) of Target: T-cell surface glycoprotein CD3 epsilon chain; T-cell surface antigen T3/Leu-4 epsilon chain; CD3e; OKT3Immunogen: Human peripheral E+ cells.
Specificity: Epitope on the epsilon-subunit within the human CD3 complex.
Antibody first published in:Kung P, Goldstein G, Reinherz EL, Schlossman SF.Monoclonal antibodies defining distinctive human T cell surface antigens.Science. 1979 Oct 19;206(4416):347-9.PMID:314668Note on publication:Describes the making of the mouse monoclonal antibodies directed against the epsilon-subunit of human CD3.
Immunofluorescence staining of fixed Molt4 cells with anti-CD3 epsilon antibody OKT-3 (Ab00122) Immunofluorescence analysis of paraformaldehyde fixed Molt4 cells on Shi-fix™ coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of OKT-3 (Ab00122-23.0) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (1 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom Ab00122-23.0, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody (Ab102-23.0) followed by Alexa Fluor® 488 secondary antibody.
