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当前位置: 首页 > 产品中心 > Functional_antibody > 绝对抗体/抗erbB-2(Her-2/neu)[4D5-8(曲妥珠单抗)]/50μg/Ab00103-19.0
商品详细绝对抗体/抗erbB-2(Her-2/neu)[4D5-8(曲妥珠单抗)]/50μg/Ab00103-19.0
绝对抗体/抗erbB-2(Her-2/neu)[4D5-8(曲妥珠单抗)]/50μg/Ab00103-19.0
绝对抗体/抗erbB-2(Her-2/neu)[4D5-8(曲妥珠单抗)]/50μg/Ab00103-19.0
商品编号: Ab00103-19.0
品牌: absoluteantibody
市场价: ¥7200.00
美元价: 4320.00
产地: 美国(厂家直采)
公司:
产品分类: 功能性抗体
公司分类: Functional_antibody
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

UniProt Accession Number of Target Protein: P04626

Alternative Name(s) of Target: Metastatic lymph node gene 19 protein (MLN 19); Proto-oncogene Neu; Proto-oncogene c-ErbB-2; Tyrosine kinase-type cell surface receptor HER2; p185erbB2; p185HER2; CD340; EGFRImmunogen: A431 cells (human epidermoid carcinoma) (over)expressing EGFR.

Specificity: Oncogenic protein Her-2/neu (erbB-2), a member of the family of epidermal growth factor (EGF) receptors, overexpressed in 30% of invasive breast cancers and 70% of ductal carcinomata in situ, in a small percentage of melanomas, and in other malignancies originating from various organs, including ovary, kidney, colon, and bladder.

Application Notes: This antibody binds to erbB-2/Her-2/neu has therapeutic indications particularly in the treatment of breast cancer (c.f. Herceptin). It can also be used to detect HER-2 by flow cytometry or IHC (Glazyrin 2007).

Antibody first published in:Carter P, Presta L, Gorman CM, Ridgway JB, Henner D, Wong WL, Rowland AM, Kotts C, Carver ME, Shepard HM.Humanization of an anti-p185HER2 antibody for human cancer therapy.Proc Natl Acad Sci U S A. 1992 May 15;89(10):4285-9.PMID:1350088Note on publication:Describes the humanisation of the antibody (also known as Herceptin), shows it recognised HER2 in a wide variety of immuno-assays.

Binding Curve
IHC on human breast tissue using Ab00103.
Binding Curve

Immunohistochemical staining of paraffin embedded human breast tissue using anti-erbB-2 antibody (Ab00103) 4D5-8 (trastuzumab) Anti-erbB-2 (Her-2/neu) staining of paraffin embedded human breast tissue using the rabbit-chimeric version of 4D5-8 (trastuzumab) (Ab00103-23.0). Antigen retreival was achieved by microwaving in citrate buffer (pH6), followed by blocking with protein block serum-free buffer (Dako, cat. #X0909). Primary antibody incubation with Ab00103-23.0 was carried out at 4 µg/ml for 30 minutes. Samples were then incubated with an anti-rabbit IgG HRP secondary antibody (Dako cat#K4009) for 20 mins followed by DAB (3,3′-diaminobenzidine), and counter-staining with haemotoxylin. Weak membrane staining exclusively of ductal epithelial cells may be observed. Recommended concentration, 4-6 µg/ml.

品牌介绍
Absolute antibody本公司服务于抗体测序、制造和工程服务提供给所有抗体使用者和不断增长的抗体。Absolute Antibody Ltd was founded in Oxford, UK, in August 2012, with the vision that all antibody users should be able to use recombinant antibodies, which are absolutely defined by their amino acid sequence. This view gained support in 2015 with a Comment in Nature magazine by Bradbury and Pluckthun (+110 co-signatories) – “Reproducibility: Standardize antibodies used in research“. Rather than creating entirely new antibodies (or antibody-like molecules), we are building a catalog by taking existing antibodies (often from hybridomas) then manufacturing them in a superior way and improving their usefulness by engineering.品牌货号名称规格absoluteantibody21 E11Anti-Api m 21 kitabsoluteantibodyBAB2Anti-Bet v 1 1 kitabsoluteantibodyBAB3Anti-Bet v 11 kitabsoluteantibodyBAB4Anti-Bet v 11 kitabsoluteantibodyBAB5Anti-Bet v 1 1 kitabsoluteantibodyBV16Anti-Bet v 1 1 kitabsoluteantibodyM0418Anti-Bet v 11 kitabsoluteantibody4C3Anti-Bla g 2 1 kitabsoluteantibody7C11Anti-Bla g 21 kitabsoluteantibodyD1Anti-Bos d 5 1 kitabsoluteantibody6A8Anti-Der f 11 kitabsoluteantibody10B9Anti-Der p 11 kitabsoluteantibody5H8Anti-Der p 11 kitabsoluteantibody4C1Anti-Neurotoxin AahII1 kitabsoluteantibodyH10Anti-Der p 21 kit