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当前位置: 首页 > 产品中心 > Functional_antibody > absoluteantibody/Ab00230-15.0 Anti-Flavivirus group antigen [D1-4G2-4-15 (4G2)]/Ab00230-15.0/50 µg
商品详细absoluteantibody/Ab00230-15.0 Anti-Flavivirus group antigen [D1-4G2-4-15 (4G2)]/Ab00230-15.0/50 µg
absoluteantibody/Ab00230-15.0 Anti-Flavivirus group antigen [D1-4G2-4-15 (4G2)]/Ab00230-15.0/50 µg
absoluteantibody/Ab00230-15.0 Anti-Flavivirus group antigen [D1-4G2-4-15 (4G2)]/Ab00230-15.0/50 µg
商品编号: Ab00230-15.0
品牌: absoluteantibody
市场价: ¥8512.00
美元价: 5107.20
产地: 美国(厂家直采)
公司:
产品分类: 功能性抗体
公司分类: Functional_antibody
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
Description: Recombinant monoclonal antibody to Flavivirus group antigen. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma D1-4G2-4-15 (4G2).
Species and Isotype: Human IgM, kappa
Clone Number: D1-4G2-4-15 (4G2)
UniProt Accession Number of Target Protein:
Published Application(s): IF; WB; NTRL; ELISA; FC; IHC-P
Published Species Reactivity: Dengue Virus; Zika Virus; West Nile Virus; Yellow Fever Virus; Flaviviridae
Immunogen: Dengue Virus type 2 antigens.
Specificity: Recognises flavivirus group specific antigens (Dengue virus, West Nile Virus, Japanese Encephalitis, Yellow Fever Virus, Zika virus etc). It binds to the fusion loop at the extremity of domain II of protein E.
Application Notes: This antibody binds to flavivirus group antigen, protein E. It can be used as an anti-Dengue virus antibody, anti-West Nile virus antibody, anti-Japanese Encephalitis, anti-Yellow Fever Virus or anti-Zika Virus antibody (Aubry et al. 2016) to identify cells infected with these flaviviridae. It binds to the fusion loop at the extremity of domain II of E protein from all four serotypes and prevents syncytia formation (Summers, 1989). The epitope is highly conserved amongst flaviviridae and has been functionally analyzed in detail by Crill and Chang 2004 (PMID: 15564505). Previous studies have used acetone- (Henchal et al. 1982) or methanol-fixed slides (Moreland & Tay, 2010). Please note that binding of this antibody has been reported to be sensitive to reduction and Western Blots should be performed under non-reducing conditions (Lai et al. 2008). A version of 4G2 directly conjugaed to APC was used by Quicke and co-workers (Quicke, 2016) to detect Zika virus in human placental macrophages by flow-cytometry (Quicke 2016). Ramaiah et al. (2016) used Ab00230-2.0 at a 1:200 dilution to stain for Zika virus in methanol-fixed Vero cells 48 h post-infection.
Antibody First Published in:
Nawa et al. Development of dengue IgM-capture enzyme-linked immunosorbent assay with higher sensitivity using monoclonal detection antibody. J Virol Methods PMID:11164919

Product Form

Purification: Affinity Purified using a recombinant lectin column
Supplied in: PBS with preservative (0.02% Proclin 300)
Storage Recommendation: Store at 4°C for up to 3 months. For longer term storage aliquot into small volumes and store at -20°C
Concentration: See vial label.

Important note – This product is for research use only. It is not intended for use in therapeutic or diagnostic procedures for humans or animals.
品牌介绍
Absolute antibody本公司服务于抗体测序、制造和工程服务提供给所有抗体使用者和不断增长的抗体。Absolute Antibody Ltd was founded in Oxford, UK, in August 2012, with the vision that all antibody users should be able to use recombinant antibodies, which are absolutely defined by their amino acid sequence. This view gained support in 2015 with a Comment in Nature magazine by Bradbury and Pluckthun (+110 co-signatories) – “Reproducibility: Standardize antibodies used in research“. Rather than creating entirely new antibodies (or antibody-like molecules), we are building a catalog by taking existing antibodies (often from hybridomas) then manufacturing them in a superior way and improving their usefulness by engineering.品牌货号名称规格absoluteantibody21 E11Anti-Api m 21 kitabsoluteantibodyBAB2Anti-Bet v 1 1 kitabsoluteantibodyBAB3Anti-Bet v 11 kitabsoluteantibodyBAB4Anti-Bet v 11 kitabsoluteantibodyBAB5Anti-Bet v 1 1 kitabsoluteantibodyBV16Anti-Bet v 1 1 kitabsoluteantibodyM0418Anti-Bet v 11 kitabsoluteantibody4C3Anti-Bla g 2 1 kitabsoluteantibody7C11Anti-Bla g 21 kitabsoluteantibodyD1Anti-Bos d 5 1 kitabsoluteantibody6A8Anti-Der f 11 kitabsoluteantibody10B9Anti-Der p 11 kitabsoluteantibody5H8Anti-Der p 11 kitabsoluteantibody4C1Anti-Neurotoxin AahII1 kitabsoluteantibodyH10Anti-Der p 21 kit