Description: Recombinant monoclonal antibody to Glycoprotein H. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybdridoma A13-d6.3.
Species and Isotype: Mouse IgG2b, kappa
Clone Number: A13-d6.3
UniProt Accession Number of Target Protein:
Published Application(s): neutralize; crystalize; immunostaining
Published Species Reactivity: pseudorabies virus (PrV)
Immunogen: d6.3 was generated by immunizing mice with PrV.
Specificity: d6.3 Fab fragment binds to the core fragment of the PrV glycoprotein H (gH) ectodomain (aa 107–639) which is the membrane-proximal end of the protein in the ?gL-Pass PrV variant of PrV. The antibody contacts residues 332-373 (peripheral helices α6 and α7 of domain III of gH contribute most of the residues that form the epitope recognized by d6.3). PrV is a porcine herpesvirus belonging to the alpha-Herpesvirinae subfamily and is known to cause Aukeszky's disease in pigs. gH is a type I membrane portein and can interact non-covalently with gL via the large gH ectodomain.
Application Notes: d6.3 neutralizes the ?gL-Pass PrV variant of PrV and can stain ?gL-Pass PrV variant but not the wild-type PrV by immunostaining. The epitope of d6.3 is not blocked by the formation of the gH/gL heterodimer. The d6.3Fab fragmnet (obtained through papain digestion) can be complexed with the core fragment of the PrV glycoprotein H (gH) ectodomain (aa 107–639) for structural studies by crystallography.
Antibody First Published in:
Backovic et al. Structure of a core fragment of glycoprotein H from pseudorabies virus in complex with antibody. Proc Natl Acad Sci U S A. 2010 Dec 28;107(52):22635-40 PMID:21149698 Note on publication: Describes the determination of the crystal structure of PrV gH complexed with d6.3 and the mode of antibody binding. The solved structure is also used to compare PrV gH with that of Epstein-Barr virus (EBV).

Product Form

Purification: Protein A affinity purified
Supplied in: PBS with preservative (0.02% Proclin 300)
Storage Recommendation: Store at 4°C for up to 3 months. For longer term storage aliquot into small volumes and store at -20°C
Concentration: See vial label.