Description: Recombinant monoclonal antibody to HBsAg. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma F124 scFv.
Species and Isotype: Mouse IgG1, kappa
Clone Number: F124 scFv
UniProt Accession Number of Target Protein:
Published Application(s): ELISA
Published Species Reactivity: Hepatitis B Virus
Immunogen: This scFv was generated from the anti-HBsAg monoclonal antibody F124, which was raised by immunization of BALB/c mice with HBV particles, serotype ay, purified from the plasma of a chronic carrier. VH and VL cDNA isolated from the F124 hybridoma were joined by a two step fusion PCR using an oligonucleotide coding for the (Gly4Ser)3 linker, and this construct was introduced into E.coli.
Specificity: This scFv is specific for the preS2 region of the surface antigen of hepatitis B virus. Specifically, this scFv recognizes an epitope localised at the N-terminal part of the M protein and containing the preS2 residue 126.
Application Notes: In ELISA analysis, this antibody has been shown to inhibit the binding of both the F124 Fab and IgG to r-HBsAg in a dose-dependent manner (Passafiume, 1998). Size-exclusion HPLC indicates that, while the scFv monomer shows no tendency to aggregate at low concentrations, equilibrium is achieved at a monomer/dimer ratio of 3:1 at high protein concentrations.
Antibody First Published in:
Passafiume et al. Sequence analysis of a monoclonal antibody specific for the preS2 region of hepatitis B surface antigen, and the cloning, expression and characterisation of its single-chain Fv construction FEBS Letters, 1998, Vol.441(3), pp.407-412 PMID:9891981 Note on publication: Describes the original generation of this scFv, and preliminary attempts to characterise its specificity and functionality.

Product Form

Purification: Protein A affinity purified
Supplied in: PBS with preservative (0.02% Proclin 300)
Storage Recommendation: Store at 4°C for up to 3 months. For longer term storage aliquot into small volumes and store at -20°C
Concentration: See vial label.