Description: Recombinant monoclonal antibody to Sigma1. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 9BG5.
Species and Isotype: Mouse IgG2a, kappa
Clone Number: 9BG5
UniProt Accession Number of Target Protein: P03528
Published Application(s): RIA; IP; FC; crystallisation
Published Species Reactivity: Reovirus strain T3D
Immunogen: Murine antibodies were produced by immunising BALB/c mice with an injection of strain TD3 reovirus. Splenocytes from immunised mice were obtained and fused with the NS1 mouse myeloma cell line to generate a hybridoma.
Specificity: 9BG5 was identified as a clone which significantly neutralised the strain T3D reovirus Sigma1 as assayed using viral titre of infection (Burstin et al, 1982). 9BG5 was able to immunoprecipitate strain T3D Sigma1, however some cross-reactivity was observed with strain T1L reoviral Sigma1 (Burstin et al, 1982; Dietrich et al, 2017). Recently, the interaction between 9BG5 and strain T3D reoviral Sigma1 was shown crystallographically (Dietrich et al, 2017). 9BG5 binds to the Sigma1 head region, with 5 CDRs contacting a relatively flat, contiguous epitope.
Application Notes: 9BG5 effectively neutralised reovirus strain T3D activity, and showed some inhibition of erythrocyte haemagglutination in virus-erythrocyte suspensions (Burstin et al, 1982). 9BG5 was also used to immunoprecipitate radiolabelled viral Sigma1 from L929 mouse fibroblast supernatants. More recently, 9BG5 was shown to cross-react with Sigma1 from the reovirus strain T1L, and contributed to blocking adherence of both T1L and T3D virions to CHO cells shown by flow cytometry (Dietrich et al, 2017).
Antibody First Published in:
Burstin et al. Evidence for functional domains on the reovirus type 3 hemagglutinin. Virology. 1982 Feb;117(1):146-55. Note on publication: Describes the generation of a panel of anti-reovirus (strain T3D) mAbs using generated murine hybridomas which recognised different functional epitopes of the viral Sigma1 protein.

Product Form

Purification: Protein A affinity purified
Supplied in: PBS with preservative (0.02% Proclin 300)
Storage Recommendation: Store at 4°C for up to 3 months. For longer term storage aliquot into small volumes and store at -20°C
Concentration: See vial label.