Description: Recombinant monoclonal antibody to Ly6G. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 1A8. This antibody was created using our proprietary Fc Silent™ engineered Fc domain containing key point mutations that abrogate binding to Fc gamma receptors.
Species and Isotype: Rat IgG2a, Fc Silent™, kappa
Clone Number: 1A8
UniProt Accession Number of Target Protein: P35461
Alternative Name(s) of Target: Ly-6G; GR-1 ; GR1; Lymphocyte antigen 6G
Published Application(s): FC; depletion
Published Species Reactivity: Mouse
Immunogen: LEW/N rats immunized with 1.5x10E8 Ly-6G-transfected EL4J cells
Specificity: Recognises the murine GPI-anchored protein Ly6G, a marker of granulocyte differentiation, maturation and, together with Ly6C, a component of the Granulocyte Receptor-1 Antigen (Gr-1). Unlike another commonly used clone, RB6-8C5, 1A8 does not bind Ly6C.
Application Notes: Neutrophils are crucial components of our immune system. Ly6G is highly expressed on neutrophils, which allows the 1A8 clone to be used not only in detection of neutrophils, but also in selective depletion in vivo. It may also be used as a negative selection marker for bone-marrow derived macrophages, which should be Ly6G(-) and F4/80 (+) (see our F4/80 range of antibodies) .
Antibody First Published in:
Fleming TJ, Fleming ML, Malek TR. Selective expression of Ly-6G on myeloid lineage cells in mouse bone marrow. RB6-8C5 mAb to granulocyte-differentiation antigen (Gr-1) detects members of the Ly-6 family. Journal of Immunology PMID:8360469 Note on publication: Describes production of 1A8 and identification of Ly6G as its target.

Product Form

Purification: Protein A affinity purified
Storage Recommendation: Store at 4°C for up to 3 months. For longer term storage aliquot into small volumes and store at -20°C
Concentration: See vial label.