Description: Recombinant monoclonal antibody to CD20. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the therapeutic antibody 10F381 (rituximab). This antibody was created using our proprietary Fc Silent™ engineered Fc domain containing key point mutations that abrogate binding to Fc gamma receptors.
Species and Isotype: Human IgG1, Fc Silent™, kappa
Clone Number: 10F381 (rituximab)
UniProt Accession Number of Target Protein: P11836
Alternative Name(s) of Target: B-lymphocyte antigen CD20; B-lymphocyte surface antigen B1; Bp35 Leukocyte surface antigen Leu-16; Membrane-spanning 4-domains subfamily A member 1; CD20
Published Application(s): FC
Published Species Reactivity: Human; Rhesus Monkey; Cynomolgus Monkey
Immunogen: Human lymphoblastoid cell line SB.
Specificity: CD20 (Human B-lymphocyte-restricted differentiation antigen, Bp35), is a hydrophobic transmembrane protein with a molecular weight of approximately 35 kDa located on pre-B and mature B lymphocytes, and most B-cell non-Hodgkin's lymphomas, but is not found on stem cells, pro-B cells, normal plasma cells or other normal tissues .
Antibody First Published in:
Reff ME, Carner K, Chambers KS, Chinn PC, Leonard JE, Raab R, Newman RA, Hanna N, Anderson DR. Depletion of B cells in vivo by a chimeric mouse human monoclonal antibody to CD20. Blood. 1994 Jan 15;83(2):435-45. PMID:7506951 Note on publication: Describes the making of the chimeric murine anti-CD20 antibody 2B8 in conjunction with human kappa and IgG1 heavy-chain constant region sequences, and analysed using flow cytometry, in vivo pharmacology and animal systemic/tissue toxicity tests.

Product Form

Purification: Protein A affinity purified
Storage Recommendation: Store at 4°C for up to 3 months. For longer term storage aliquot into small volumes and store at -20°C
Concentration: See vial label.