Description: Recombinant monoclonal antibody to CD4 . Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma CE9.1 (Clenoliximab). This antibody was created using our proprietary Fc Silent™ engineered Fc domain containing key point mutations that abrogate binding to Fc gamma receptors.
Species and Isotype: Mouse IgG1, Fc Silent™, lambda
Clone Number: CE9.1 (Clenoliximab)
UniProt Accession Number of Target Protein: P01730
Alternative Name(s) of Target: p55; T cell surface antign T4/Leu-3.
Published Application(s): ELISA; FC; Block; SPR; IHC
Published Species Reactivity: Human; chimpanzee
Immunogen: Domain 1 of human CD4.
Specificity: The antibody binds to human CD4 with a Kd of 1.0 nM and an ED50 of 0.2 ug/ml.
Application Notes: The antibody binds specifically to CD4, a molecule which is part of the immunolgobulin super-family, expressed on the surface of helper T cells. The CD4 molecule acts as a co-receptor in the activation of T cells, aiding the interaction between the TCR and MHC II of the antigen-presenting cell. The antibody is classified as 'primatized', as the variable regions of the light and heavy chains are obtained from primates, while the lamda light chain and γ4 heavy chain constant domains are human. The antibody was engineered from the primatized IgG1 monoclonal antibody Keliximab by substituting two key residues. The role of the antibody as an immunosuppressor has been tested in vivo, by intravenous administration in chimpanzees and human volunteers. The antibody reached Phase II clinical trials with Biogen-Idec.
Antibody First Published in:
Newman et al. Primatization of recombinant antibodies for immunotherapy of human diseases: a macaque/human chimeric antibody against human CD4. Biotechnology (NY). 1992 Nov;10(11):1455-60. PMID:1369023 Note on publication: Describes the method of producing 'primatized' antibodies, where immunoglobulin genes from cynomolgus monkeys immunised with human CD4 are isolated, amplified and then cloned. The antibodies generated are against the CD4 molecule expressed on the surface of helper T cells, and are tested for both in vitro and in vivo use.

Product Form

Purification: Protein A affinity purified
Storage Recommendation: Store at 4°C for up to 3 months. For longer term storage aliquot into small volumes and store at -20°C
Concentration: See vial label.