Description: Recombinant monoclonal antibody to CD40L. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma hu5c8 (Ruplizumab). This antibody was created using our proprietary Fc Silent™ engineered Fc domain containing key point mutations that abrogate binding to Fc gamma receptors.
Species and Isotype: Mouse IgG1, Fc Silent™, kappa
Clone Number: hu5c8 (Ruplizumab)
UniProt Accession Number of Target Protein: P29965
Alternative Name(s) of Target: CD40 ligand; tumour necrosis factor ligand superfamily member 5; TNFSF5; CD154; tumour necrosis factor related activation protein; TRAP.
Published Application(s): Block; FC; WB
Published Species Reactivity: Human
Immunogen: Human CD40L
Specificity: The antibody binds to CD40L with an IC50 of 0.845 ug/ml.
Application Notes: The antibody binds specifically to CD40L, a surface receptor expressed on activated T cells which acts as a costimulatory molecule to trigger immune responses. The antibody neutralises CD40L function, as it blocks the interaction between CD40 and CD40L. When injected into cynomolgus monkeys, the original therapeutic antibody was found to have a T1/2 of 531? ±?155 hours.
Antibody First Published in:
Karpusas et al. Structure of CD40 Ligand in Complex with the Fab Fragment of a Neutralizing Humanized Antibody Structure. 2001 Apr 4;9(4):321-9. PMID:11525169 Note on publication: Describes the role of CD40-CD40L interaction in immune responses and pathologies. Also describes the generation of humanized monoclonal antibodies against CD40L and the use of a crystal structure to determine its binding.

Product Form

Purification: Protein A affinity purified
Storage Recommendation: Store at 4°C for up to 3 months. For longer term storage aliquot into small volumes and store at -20°C
Concentration: See vial label.