Description: Recombinant monoclonal antibody to IL-6 receptor. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the therapeutic antibody rhPM-1 (Tocilizumab).
Species and Isotype: Human IgG1, kappa
Clone Number: rhPM-1 (Tocilizumab)
UniProt Accession Number of Target Protein: A0N0L5
Published Application(s): ELISA; inhibit
Published Species Reactivity: human
Immunogen: This antibody was prepared by CDR grafting of mouse AUK 12-20 antibody onto a human framework regions.
Specificity: Tocilizumab binds specifically to both the membrane bound and soluble forms of human IL-6R. The IL6 receptor is a protein complex consisting of an IL-6 receptor subunit (IL6R) and interleukin 6 signal transducer Glycoprotein 130. Interleukin 6 (IL6) is a potent pleiotropic cytokine that regulates cell growth and differentiation and plays an important role in immune response.
Application Notes: Tocilizumab can be used in ELISA assays and is able to inhibit the growth of IL-6-dependent tumor cell lines (to the same extent as the original AUK12-20 mouse antibody) - inhibits sIL-6R and mIL-6R-mediated signaling.
Antibody First Published in:
Sato et al. Humanization of a mouse anti-human interleukin-6 receptor antibody comparing two methods for selecting human framework regions. Mol Immunol. 1994 Apr;31(5):371-81. PMID:8152440 Note on publication: Describes the design and construction of reshaped human AUKl2-20 antibodies which bind specifically to human IL-6R.

Product Form

Purification: Protein A affinity purified
Storage Recommendation: Store at 4°C for up to 3 months. For longer term storage aliquot into small volumes and store at -20°C
Concentration: See vial label.